The transcriptional activator HIyU of Vibrio cholerae: nucleotide sequence and role in virulence gene expression
- 1 August 1993
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 9 (4) , 751-760
- https://doi.org/10.1111/j.1365-2958.1993.tb01735.x
Abstract
HlyU upregulates expression of the haemolysin, HlyA, of Vibrio cholerae. DNA sequence analysis indicates that HlyU is an 11.9 kDa protein containing a putative helix-turn-helix motif and belonging to a family of small regulatory proteins, including NolR (Rhizobium meliloti), SmtB (Synechococcus PCC 7942) and ArsR (piasmids R773, Escherichia coli; p1258, Staphylococcus aureus; and pSX267, Staphylococcus xylosus). An hlyU mutant was constructed by insertional inactivation, and found to be deficient in the production of both the haemolysin and a 28kDa secreted protein. The mutant was assessed for virulence in the infant mouse cholera model, revealing a 100-fold increase in the LD50.. This suggests that HlyU promotes expression of virulence determinant(s) in vivo.Keywords
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