Detection of multiple β‐casein (CASB) alleles by amplification created restriction sites (ACRS)
- 1 August 1992
- journal article
- Published by Wiley in Animal Genetics
- Vol. 23 (4) , 333-338
- https://doi.org/10.1111/j.1365-2052.1992.tb00155.x
Abstract
Summary. Direct sequencing of polymerase chain reaction (PCR) amplified DNA has been used to detect the DNA sequences for bovine β‐casein (CASB) A3 and B variants. Based on these sequences we have designed primers which create allele‐specific restriction sites in the PCR product. Restriction analysis of PCR product generated in one reaction enable us to identify the A1, A2, A3 and B alleles of CASB rapidly without the use of radioactivity.Keywords
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