Mapping of the Rsd Contact Site on the Sigma 70 Subunit of Escherichia coli RNA Polymerase
Open Access
- 1 May 2001
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (9) , 3318-3327
- https://doi.org/10.1128/jb.183.9.2952-2956.2001
Abstract
Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase ς 70 subunit. The contact site of Rsd on ς 70 was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron- p -bromoacetamidobenzyl EDTA and by analysis of the complex formation between Ala-substituted ς 70 and Rsd. Results indicate that the Rsd contact site is located downstream of the promoter −35 recognition helix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core enzyme and ς 70 contact transcription factors.Keywords
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