Frequency and distribution of nuclear pores in Cucurbita maxima cotyledons as revealed by freeze-etching

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Abstract
The frequency and distribution of nuclear pores in the cotyledon mesophyll cells of Cucurbita maxima were studied during germination. During the early stages of seed germination the nuclear envelope of the mesophyll cells was found to contain a distinctive pattern of nuclear pores. The nuclear pores were in short rows that divided the nuclear membrane into areas in which pores were absent. Mesophyll cells from a later stage of germination contained nuclear envelopes with significantly more nuclear pores per unit area. At the later stage of germination the distinctive pattern of nuclear pores was less readily distinguishable by visual observation of electron micrographs, but pattern analysis revealed that the pattern was still present.