The involvement of histone H1° in chromatin structure
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (8) , 2843-2853
- https://doi.org/10.1093/nar/13.8.2843
Abstract
Micrococcal nuclease digestion and light scattering are used to compare native chromatins with various histone H1° contents. The experimental data show that the higher the H1° content, the greater the ability to form compact structures with increasing ionic strength, and the lower the DNA accessibility to micrococcal nuclease. On the contrary, reconstituted samples from H1-depleted chromatin and pure individual H1 fractions behave in such a way that samples reconstituted with pure H1° give rise to a looser structure, more accessible to nuclease than samples reconstituted with H1-1.Keywords
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