Cytochrome P450-Dependent Arachidonic Acid Metabolites, 19− and 20-Hydroxyeicosatetraenoic Acids, Enhance Sodium-Potassium ATPase Activity in Vascular Smooth Muscle

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Abstract
Several cytochrome P450-dependent arachidonic acid metabolites have been shown to affect Na+,K+-ATPase activity. In the present study, we tested the effect of α and ω - 1-hydroxylated products, i.e., 19− and 20-hydroxyeicosatetraenoic acids (19− and 20-HETE), on the K-induced relaxation in rat aortic rings. 19-HETE and 20-HETE increased the magnitude of the potassium-induced relaxation in a dose-dependent fashion (10−7-10−5M). The inhibitory effect of ouabain on the potassium-induced relaxation was reversed by both 19− and 20-HETE. In addition, indomethacin fully inhibited the stimulatory effect of 19− and 20-HETE on relaxation induced by potassium. Vascular ouabainsensitive 86Rb uptake was also increased by 19− and 20-HETE. These observations suggest that 19− and 20-HETE stimulate vascular Na+,K+-ATPase via their conversion by cyclooxygenase to prostaglandin-like material.