Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.
- 1 December 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (24) , 7447-7451
- https://doi.org/10.1073/pnas.80.24.7447
Abstract
A small nucleotidyl protein was synthesized in vitro in a membrane fraction of poliovirus-infected HeLa [human cervical carcinoma] cells. Analyses of the nucleotides and polypeptide showed that the nucleotidyl protein is VPg-pUpU, the genome-linked protein of poliovirion RNA covalently bound to the first 2 5''-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. Evidently, VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.This publication has 35 references indexed in Scilit:
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