Collagen of fibrocartilage: a distinctive molecular phenotype in bovine meniscus
- 25 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (2) , 265-270
- https://doi.org/10.1016/0014-5793(83)80592-4
Abstract
Fibrocartilage of bovine knee meniscus was analyzed for major and minor collagen constituents. The main fraction (∼98%) of pepsin-solubilized collagen consisted of type I with a small proportion (< 10%) of type III molecules. The minor fraction (1–2%) isolated by salt precipitation could be further resolved into type V collagen that consisted of α1(V) and α2(V) chains and a type II-like molecule with chains that had all the characteristics of the 3α variant of α1(II) found in hyaline cartilage. The articular surface zone of the meniscus appeared 2–3-fold enriched in these minor collagens compared with deeper tissue, though qualitatively the same distinctive collagen phenotype was evident throughout.Keywords
This publication has 13 references indexed in Scilit:
- Marfan syndrome: abnormal alpha 2 chain in type I collagen.Proceedings of the National Academy of Sciences, 1981
- Isolation and characterisation of an unusual collagen from hyaline cartilage and intervertebral discFEBS Letters, 1981
- Identification of a new disulphide bonded collagen from cartilageFEBS Letters, 1980
- Collagen heterogeneity in human cartilage: Identification of several new collagen chainsBiochemical and Biophysical Research Communications, 1979
- Physicochemical characterization and molecular organization of the collagen A and B chainsBiochemistry, 1978
- Fetal membrane collagens: identification of two new collagen alpha chains.Proceedings of the National Academy of Sciences, 1976
- Rapid visuallzation of protein-dodecyl sulfate complexes in polyacrylamide gelsAnalytical Biochemistry, 1976
- The distribution of different molecular species of collagen in fibrous, elastic and hyaline cartilages of the pigBiochemical Journal, 1975
- Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsinBiochemistry, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970