DIFFERENTIAL DEPLETION OF LYMPHOCYTES-T IN THE SPLEEN OF DENGUE VIRUS-INFECTED MICE

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 37  (1) , 1-6
Abstract
Following the i.c. [intracutaneous] inoculation of dengue type 2 virus (DV) the spleen wt of infected mice was reduced, as was the proportion of cells killed by ATS [anti-thymocyte serum] and complement (T [thymus-derived] lymphocytes) in spleen cell suspensions. In DV-infected mice the mean hemolysin titer 16 days after i.p. inoculation of 4 .times. 108 SRBC [sheep red blood cells] was 47 compared with 406 in normal mice. Spleen cells from DV-infected mice produced significantly reduced direct GVH [graft vs. host] reactivity in Parker strain (PS) infant mice. Adoptive transfer of spleen cells obtained from mice given 3 weekly i.p. doses of DV or a single i.c. dose suppressed antigen-specific antibody secretion as detected by the Jerne plaque technique. This suppression was abrogated by pre-treating the transferred cells with ATS and complement. DV selectively depletes T lymphocyte subpopulations responsible for helper and effector functions and spares suppressor T cells in the spleen of infected mice.