Macropinocytosis as a Mechanism of Entry into Primary Human Urethral Epithelial Cells by Neisseria gonorrhoeae
Open Access
- 1 March 2000
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (3) , 2435-2440
- https://doi.org/10.1128/iai.68.3.1696-1699.2000
Abstract
Gonococcal entry into primary human urethral epithelial cells (HUEC) can occur by macropinocytosis. Scanning and transmission electron microscopy revealed lamellipodia surrounding gonococci, and confocal laser scanning microscopy analysis showed organisms colocalized with M r 70,000 fluorescein isothiocyanate-labeled dextran within the cells. Phosphoinositide 3-kinase inhibitors and an actin polymerization inhibitor prevented macropinocytic entry of gonococci into HUEC.Keywords
This publication has 9 references indexed in Scilit:
- Enteropathogenic E. coli, Salmonella, and Shigella: Masters of Host Cell Cytoskeletal ExploitationEmerging Infectious Diseases, 1999
- A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.The Journal of cell biology, 1996
- The Pathogenesis of Gonococcal Urethritis in Men: Confocal and Immunoelectron Microscopic Analysis of Urethral Exudates from Men Infected with Neisseria gonorrhoeaeThe Journal of Infectious Diseases, 1996
- MacropinocytosisTrends in Cell Biology, 1995
- Salmonella invasion of nonphagocytic cells induces formation of macropinosomes in the host cellInfection and Immunity, 1994
- Ruffles induced by Salmonella and other stimuli direct macropinocytosis of bacteriaNature, 1993
- Model for invasion of human tissue culture cells by Neisseria gonorrhoeaeInfection and Immunity, 1988
- Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulationInfection and Immunity, 1987
- [33] Use of horseradish peroxidase and fluorescent dextrans to study fluid pinocytosis in leukocytesPublished by Elsevier ,1984