Two‐step fermentation process for improved xanthan production by Xanthomonas campestris NRRL‐B‐1459

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Abstract
Xanthan formation by Xanthomonas campestris NRRL‐B‐1459 was optimised in a two‐step fermentation cycle. In conventional batch fermentation a cell growth phase was followed by a xanthan production phase. This was replaced by a two‐step process, comprising an initial biomass formation step followed by a xanthan formation step that required a high C/N ratio and higher aeration and agitation levels. The optimal moment of process change was critical and occurred 25–30 h after inoculation; at this time the contents of one biomass production fermenter could then be subdivided to feed 10 xanthan production tanks. Relatively high levels of residual sucrose remained even after the completion of xanthan formation.

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