Microcalorimetric Determination of Glucose in Reference Samples of Serum

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Abstract
I report a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. the method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by spectrophotometric hexokinase/glucose-6-phosphate dehydrogenase method and by isotope-dilution mass spectrometry. In most cases, the microcalorimetric results appear to be in agreement with the results obtained by these latter two method. A discussion of the basis of the mocrocalorimetric measurments is presented.

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