Identification of the disulphide bonds in human platelet glycocalicin
- 1 July 1991
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 199 (2) , 389-393
- https://doi.org/10.1111/j.1432-1033.1991.tb16135.x
Abstract
The glycoprotein Ib/IX complex on platelets is responsible for the first stage of haemostasis as an essential component in the primary adhesion of platelets to damaged vessel walls. Glycocalicin is the extracellular part of platelet glycoprotein Ib alpha and contains the von Willebrand factor and thrombin binding sites. Disulphide bonds are implicated in the von Willebrand binding site and studies with peptides point towards a region of glycocalicin with four cysteines as containing the binding sites for both von Willebrand factor and thrombin. The position and linkage of these two disulphide bonds are now determined to be 209-248 and 211-264 and the relevance of this double-loop structure for glycoprotein Ib/IX function is discussed.Keywords
This publication has 28 references indexed in Scilit:
- The Interaction of von Willebrand Factor and the Platelet Glycoprotein Ib-IX ComplexPlatelets, 1990
- Purification of botrocetin from Bothrops jararaca venom. Analysis of the botrocetin-mediated interaction between von Willebrand factor and the human platelet membrane glycoprotein Ib-IX complexBiochemistry, 1989
- Cross-linking of a monomeric 39/34-kDa dispase fragment of von Willebrand factor (Leu-480/Val-481-Gly-718) to the N-terminal region of the .alpha.-chain of membrane glycoprotein Ib on intact platelets with bis(sulfosuccinimidyl) suberateBiochemistry, 1989
- Identification of disulfide-bridged substructures within human von Willebrand factorBiochemistry, 1987
- The glycoprotein Ib complex of human blood plateletsEuropean Journal of Biochemistry, 1987
- Monoclonal antibody to glycoprotein Ib inhibits both thrombinand ristocetin-induced platelet aggregationsThrombosis Research, 1985
- Structural studies on the O-linked carbohydrate chains of human platelet glycocalicinEuropean Journal of Biochemistry, 1984
- ROLE OF VON WILLEBRAND FACTOR IN PLATELET ADHESION TO THE SUBENDOTHELIUMBritish Journal of Haematology, 1983
- Properties of human asialo-factor VIII. A ristocetin-independent platelet-aggregating agent.Journal of Clinical Investigation, 1981
- Platelet glycocalicin: A single receptor for platelet aggregation induced by thrombin or ristocetinThrombosis Research, 1976