A new method in criminology: Use of ELISA to detect AFP on different materials with monoclonal anti-?-fetoprotein
- 1 November 1984
- journal article
- research article
- Published by Springer Nature in International journal of legal medicine
- Vol. 93 (2) , 117-121
- https://doi.org/10.1007/bf00200770
Abstract
A new method has been introduced to distinguish normal adult serum stains from fetal or newborn serum and amniotic fluid stains with ELISA in cases of criminal abortion and infanticide. The method is based on the sensitive detection of α-fetoprotein (AFP) by a two-site enzyme immunoassay (EIA) following its elution with high efficiency from different materials (e.g., cotton, paper, synthetic fabric, or glass) by phosphate-buffered 0.5 M NaCl solution Es wurde eine Methode erarbeitet, um in Fällen von Abtreibung bzw. Kindestötung (Tötung des Neugeborenen) zwischen den vom Neugeborene herrührenden biologischen Spuren (Blut- und Fruchtwasserflecke) und den von anderen Individuen stammenden Blutflecken unterscheiden zu können. Von der Oberfläche der verschiedenen Spurenträger (Baumwollstoff, Papier, Kunstfaser und Glas) wurden die eingetrockneten Flecke mit 0,5 M NaCl enthaltendem Phosphatpuffer eluiert und dann aus den Eluaten das Alpha-Fetoprotein mit monoklonalen Antikörpern mit Hilfe der ELISA-Technik nachgewiesen.Keywords
This publication has 10 references indexed in Scilit:
- Monoclonal antibodies to human alphafetoproteinMolecular Immunology, 1980
- Maternal serum-alpha-fetoprotein measurement in antenatal screening for anencephaly and spina bifida in early pregnancy. Report of U.K. collaborative study on alpha-fetoprotein in relation to neural-tube defects.1977
- ORGANISATION OF MATERNAL SERUM-α-FETOPROTEIN SCREENING FOR FETAL NEURAL-TUBE DEFECTSThe Lancet, 1977
- Serum alpha-fetoprotein in adults, in women during pregnancy, in children at birth, and during the first week of life: A sex differenceAmerican Journal of Obstetrics and Gynecology, 1977
- Double-antibody enzyme immunoassay applied to human alpha 1-fetoprotein.Clinical Chemistry, 1976
- α-Foetoprotein in Normal Human SerumNature, 1972
- Studies of carcino-fetal proteins: Physical and chemical properties of human α-fetoproteinInternational Journal of Cancer, 1971
- Sites of Serum α-Fetoprotein Synthesis in the Human and in the Rat*Journal of Clinical Investigation, 1967
- Serum alpha-fetoprotein, albumin, and gamma-G-globulin in the human conceptus.Journal of Clinical Investigation, 1966
- Antigenic Structure of Chemically-Induced HepatomasPublished by S. Karger AG ,1965