Fast liquid chromatography coupled to electrospray tandem mass spectrometry peptide sequencing for cross‐species protein identification
Open Access
- 29 August 2003
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 17 (19) , 2188-2194
- https://doi.org/10.1002/rcm.1173
Abstract
Using a parallel microcolumn switching liquid chromatography set-up coupled to a quadrupole time-of-flight mass spectrometer, a rapid liquid chromatography/mass spectrometric (LC/MS) protein identification method is presented. Without prior sample clean-up up to 300 protein digest samples a day can be processed. Using data-directed acquisition, up to 10 fragmentation analyses for each protein sample can be acquired in the same chromatographic run that can be used for database searching. Using internal peptide sequence information, protein databases and the various nucleic acid databases can both be queried for cross-species identification of the protein sample. The method was evaluated and put into force to generate data for a tobacco cell culture protein database. Copyright © 2003 John Wiley & Sons, Ltd.Keywords
This publication has 7 references indexed in Scilit:
- Expanding the organismal scope of proteomics: Cross‐species protein identification by mass spectrometry and its implicationsProteomics, 2003
- Charting the Proteomes of Organisms with Unsequenced Genomes by MALDI-Quadrupole Time-of-Flight Mass Spectrometry and BLAST Homology SearchingAnalytical Chemistry, 2001
- The current state of two-dimensional electrophoresis with immobilized pH gradientsElectrophoresis, 2000
- Probability-based protein identification by searching sequence databases using mass spectrometry dataElectrophoresis, 1999
- Cross-Species Protein Identification using Amino Acid Composition, Peptide Mass Fingerprinting, Isoelectric Point and Molecular Mass: A Theoretical EvaluationJournal of Theoretical Biology, 1997
- Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide GelsAnalytical Chemistry, 1996
- Tobacco BY-2 Cell Line as the “HeLa” Cell in the Cell Biology of Higher PlantsPublished by Elsevier ,1992