Amplification of Mycoplasma iowae Using Polymerase Chain Reaction
- 1 January 1993
- journal article
- Published by JSTOR
- Vol. 37 (1) , 212-7
- https://doi.org/10.2307/1591476
Abstract
Based on sequence data of Mycoplasma iowae recombinant DNA probe, pMI-12, two 25-base primers were synthesized for use in the polymerase chain reaction (PCR). An M. iowae species-specific 299-base pair product was amplified by the primers. An annealing temperature of 58.5 C was critical for detecting all members of this heterogeneous species while maintaining specificity of the M. iowae PCR. The minimum amount of target DNA detected by M. iowae PCR was 1 pg, which was 1000 times more sensitive than the dot-blot assay using M. iowae recombinant DNA probes.Keywords
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