Expression of the vasopressin and oxytocin genes in human hypothalami
- 12 November 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 193 (1) , 12-16
- https://doi.org/10.1016/0014-5793(85)80069-7
Abstract
Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the polyprotein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immunoprecipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro-vasopressin). A second less intense product of Mr 16500 was tentatively identified as prepro-oxytocin. A cDNA library derived from the human hypothalamic poly(A)+ RNA was screened for vasopressin and oxytocin-encoding cDNA using heterologous probes; clones encoding the two precursors were identified and found to be organized as their rat and bovine counterparts. Northern blot analysis shows that the mRNAs for the two prepro-hormones consist of ~ 840 (AVP) and ~ 700 (OT) nucleotidesKeywords
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