UPTAKE AND TRANSLOCATION OF C14-LABELLED SUGARS APPLIED TO PRIMARY LEAVES OF SOYBEAN SEEDLINGS

Abstract
A method involving the use of a detergent and boron has been developed for the introduction of C14-labelled sugars into leaves. This method was used for translocation studies.In the light, translocation of sucrose-C14 or glucose-C14 out of the treated leaf was very slow. Only 1% of the C14 from sucrose was translocated after 14 hours. In the dark, 10% of the C14 from glucose was translocated to all parts of the seedling in 3 hours. The bulk of the translocated C14 accumulated in the stem between the treated leaf and the root. The actively growing stem tip accumulated very little C14 in either light or dark.In light or dark, C14 from a primary leaf that had been allowed 15 minute photosynthesis in C14O2 spread throughout the entire plant in 3 hours. In light, more C14 from photosynthate accumulated in the growing stem tip than in the root. This situation was reversed in the dark.

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