Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12
- 31 May 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 134 (3) , 1117-1122
- https://doi.org/10.1128/jb.134.3.1117-1122.1978
Abstract
Replicating DNA molecules of the nonconjugative F plasmid RSF1010 (Smr [streptomycin resistance] Sur [sulfonamide resistance] were cleaved with the EcoRI restriction endonuclease and examined by EM. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from 1 of the EcoRI ends. Replication proceeded unidirectionally or bidirectionally with equal frequency. Results of the analysis of replicative intermediates of RSF1010 containing the Apr[ampicillin resistance]-transposable sequence (Tn) are also presented.This publication has 28 references indexed in Scilit:
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