Extracellular matrix components produced by SV40-transformed human epidermal keratinocytes

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Abstract
The production of fibronectin, laminin and glycosaminoglycans (GAGs) has been investigated in normal and SV40‐transformed human epidermal keratinocytes. Normal keratinocytes produced small amounts of laminin and fibronectin and three classes of GAGs; hyaluronic acid, heparan sulphate and chondroitin sulphate, hyaluronic acid being the major GAG. The SV40‐transformed keratinocytes retained their cell‐surface laminin and fibronectin, and in some cases showed elevated levels when compared to normal keratinocytes. All the transformants showed a shift in GAG production from hyaluronic acid to heparan sulphate, so that in all SV40‐transformed keratinocytes heparan sulphate was the major GAG. These results demonstrate that the loss of extracellular matrix, which normally occurs in transformed fibroblastic cells, is not a feature of SV40‐transformed human keratinocytes.