IDENTIFICATION AND PURIFICATION OF SPECIFIC ANTIGEN OF PARACOCCIDIOIDES-BRASILIENSIS RESPONSIBLE FOR IMMUNOELECTROPHORETIC BAND E

  • 1 January 1977
    • journal article
    • research article
    • Vol. 15  (MAR) , 79-85
Abstract
A new [serodiagnostic] purified antigen (E2] of P. brasiliensis mycelial growth phase was isolated by immunoadsorption from a crude metabolic soluble extract of the fungus. The antiserum prepared in a rabbit by inoculation of E2 antigen developed only 1 immunodiffusion line with the crude metabolic extract. Findings on immunological analysis showed that E2 antigen is the antigenic component of immunoelectrophoretic band E. The isolated antigens did not possess detectable alkaline phosphatase activity. It reacted in immunodiffusion tests with all the sera (14/14) from P. brasiliensis infected patients containing precipitating antibodies.