Levels of Disialogangliosides in Sera of Melanoma Patients Monitored by Sensitive ThinLayer Chromatography and Immunostaining
- 4 October 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 81 (19) , 1489-1492
- https://doi.org/10.1093/jnci/81.19.1489
Abstract
Levels of GD2, GD3, and 9-O-acetyl GD3 were monitored in sera of patients with melanoma and healthy adults with two monoclonal antibodies that specifically detect these gangliosides. By direct measurement of radioactivity in the im-munolabeled chromatogram, GD2 could be detected in normal sera at 2 ng/mL. Serum levels of GD2 and GD3 were increased approximately sixfold and fivefold, respectively, in patients with disseminated melanoma, compared with those of healthy adults. The acetylated derivative of GD3, which is highly specific for melanoma cells, was not detected in serum. This sensitive assay allows the quantitation of tumor-associated gangliosides that are circulating in sera of melanoma patients. [J Natl Cancer Inst 81: 1489–1492, 1989]This publication has 1 reference indexed in Scilit:
- Proton NMR and fast-atom bombardment mass spectrometry analysis of the melanoma-associated ganglioside 9-O-acetyl-GD3.Journal of Biological Chemistry, 1985