HLA‐B*35–Restricted CD8 T Cell Epitopes in the Antigen 85 Complex ofMycobacterium tuberculosis

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Abstract
Few target epitopes have been described for human CD8 T lymphocytes in antigens of Mycobacterium tuberculosis By use of a reverse immunogenetics approach, 23 motif-bearing peptides of the Ag85 complex were tested for binding to HLA-B*35, one of the common B-types in West Africa. Three 9-mer peptides bound with high affinity to HLA-B*3501 and displayed low dissociation rates of peptide–major histocompatibility complexes (MHCs). IC50 and half-life values of peptide–MHC class I complexes were in the same range as reported earlier for other immunogenic peptides. Immune responses against peptide Ag85C (aa 204–212) WPTLIGLAM were characterized in detail. Peptide-stimulated effector cells were able to kill macrophages infected with M. tuberculosis or bacille Calmette-Guérin. Peptide-specific CD8 T cells could be visualized by using HLA-B*3501 tetramers and were shown to produce interferon-γ and tumor necrosis factor–α. Together with other published epitopes, these peptides can be used to study more closely the role of CD8 T cells in mycobacterial infection and tuberculosis

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