Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens
- 1 June 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (6) , 2280-2
- https://doi.org/10.1128/jcm.39.6.2280-2282.2001
Abstract
The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae- positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively.Keywords
This publication has 18 references indexed in Scilit:
- Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic Acid Amplification TestsJournal of Clinical Microbiology, 2001
- Multicenter Evaluation of AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Neisseria gonorrhoeaeJournal of Clinical Microbiology, 2000
- Evaluation of AMPLICOR Neisseria gonorrhoeae PCR Using cppB Nested PCR and 16S rRNA PCRJournal of Clinical Microbiology, 1999
- Evaluation of the Abbott LCx Assay for Detection of Neisseria gonorrhoeae in Endocervical Swab Specimens from FemalesJournal of Clinical Microbiology, 1998
- Evaluation of the Abbott LCx Ligase Chain Reaction Assay for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Urine and Genital Swab Specimens from a Sexually Transmitted Disease Clinic PopulationJournal of Clinical Microbiology, 1998
- Evaluation of 2-SP transport medium for detection of Chlamydia trachomatis and Neisseria gonorrhoeae by two automated amplification systems and culture for chlamydia.Journal of Clinical Pathology, 1997
- Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assayJournal of Clinical Microbiology, 1997
- Ligase chain reaction for detection of Neisseria gonorrhoeae in urogenital swabsJournal of Clinical Microbiology, 1995
- Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimensJournal of Clinical Microbiology, 1995
- Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples.Journal of Clinical Pathology, 1992