Transformation of mouse cells after infection with ultraviolet irradiation‐inactivated herpes simplex virus type 2

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Abstract
A transformed mouse cell line (H238) was obtained following the infection of 238 mouse cells with ultraviolet (UV) irradiation‐inactivated herpes simplex virus type 2 (HSV‐2). The transformed cells produced tumors with a 100% incidence within 8 weeks in 6‐week‐old syngeneic BALB/c mice at an inoculum of 1 × 106. Indirect immunofluorescence (IF) tests revealed the presence of HSV antigens in the transformed cells. Antibodies to HSV‐2 were found in the sera of tumor‐bearing animals by neutralization and IF techniques. Neither HSV‐2 infectious virus nor viral antigens could be detected by the transfer of transformed‐cell DNA into permissive cells.