Japanese B Encephalitis Virus in Tissue Culture.

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Abstract
Two strains of Japanese B encephalitis (JBE) virus were propagated serially in tissue cultures of chick-embryo fibroblasts, monkey kidney epithelium, HeLa cells, and Detroit-6 cells. Cyto-pathogenicity which was consistent and reproducible was observed only with the Detroit-6 cell line. Maximal virus growth (105 to 106 mouse LD 50/ml of tissue culture fluid) was seen 2 days before cytopathogenicity became pronounced. The cytopathogenicity was never 100% complete and cell sheets were observed to recover and virus proliferation continue when nutrient fluids were regularly replaced. Cytopathogenicity was readily neutralized by JBE immune serum. A similar cytopathogenic effect was observed in Detroit-6 cells infected with the Egypt 101 strain of West Nile virus.