Ultrastructural Changes in in vitro Ageing Spinach Chloroplasts

Abstract
Ultrastructural changes in in vitro aging spinach chloroplasts were studied in detail. Prolonged storage caused swelling of the chloroplasts due to the increase in the thickness and spacing of the thylakoid membranes. The increase in the thickness of the membrane is partly accompanied by the release of lipids. Addition of crystalline bovine serum albumin stabilized the membrane structures. Storage of the chloroplasts at 77.degree. K, even though it resulted in complete breakage of the whole chloroplast, maintained the thylakoid structures in a highly intact form.

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