The cytochemical reactivity of cerium ions with cardiac muscle.

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Abstract
When unfixed cardiac muscle from the rat or hamster was incubated in substrate-free media containing cerous chloride buffered to pH 7.5 with tris-maleate-sucrose, electron-dense reaction bodies were formed in the sarcoplasma and, to a greater extent, inside and budding from mitochondria. Fixation in glutaraldehyde prevented the reaction product appearing within mitochondria, but had no effect on either the bodies associated with outer mitochondrial membranes or those apparently free in the sarcoplasm or outside the cells. No reaction product was observed in unfixed liver, kidney or skeletal muscle. Apparently, the electron-dense product arises from a reaction of Ce ions with either a lipid peroxide or endogenous H2O2 generated by a metal-containing thiol enzyme, possibly an oxidized form of monoamine oxidase A with the properties of a diamine oxidase. The significance of the Ce reaction in the aging of cells is discussed briefly.