The outer layer of the cell envelope of Halobacterium halobium

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Abstract
The outer layer of the cell envelope of Halobacterium halobium was isolated after suspending the envelope in either 1 M NaCl or 0.02 M MgCl2. Chemical analysis of the isolated, solubilized outer layer showed it to consist of protein or glycoprotein with about 3% RNA. No free or bound lipid was detected. No cytochromes were present in the outer layer. Components commonly associated with bacterial cell walls were absent.Chemical composition together with the marked instability of the outer layer in a slight ion deficit are not consistent with a function of this layer as a "cell wall" of the organism.

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