Tritiation of α-bungarotoxin with N-succinimidyl [2,3-3H]propionate. A useful reagent for labelling proteins
- 1 March 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 193 (3) , 919-923
- https://doi.org/10.1042/bj1930919
Abstract
Mono[3H]propionyl-alpha-bungarotoxin, prepared with N-succinimidyl [2,3-3H]propionate (sp. radioactivity 50 Ci/mmol) and purified to homogeneity by electrofocusing, retains its biological activity and stability. Rate constants for its binding to acetylcholine receptor were 4.4-fold lower than for unlabelled toxin; no dissociation was detectable. Analysis of enzymic digests of toxin showed 3H is located mainly of entirely in epsilon-propionyl-lysine.This publication has 10 references indexed in Scilit:
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