High-density screen of human tumor cell lines for homozygous deletions of loci on chromosome arm 8p

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Abstract
Tumor cell‐specific homozygous deletions coinciding at a particular genetic location may indicate the inactivation of a nearby tumor suppressor gene. Forty‐six human cancer cell lines of prostate, pancreatic, lung, liver, and colon origin were screened for homozygous deletions of 139 expressed sequence tag (EST) and sequence‐tagged site (STS) loci spanning the entire short arm of chromosome 8. Only one Southern blot–verified homozygous deletion was detected in this set of cell lines. The deletion, in pancreatic tumor cell line MIA‐PaCa‐2, encompassed two screening loci, D8S549 and D8S1992, and overlapped another previously described homozygous deletion of band 8p22 in a metastatic prostate cancer specimen. Both deletions entirely removed the candidate tumor suppressor gene N33. These data define a consensus homozygous deletion region in chromosome band 8p22. Genes Chromosomes Cancer 24:42–47, 1999.