INVIVO DOSIMETRY OF 4-AMINOBIPHENYL IN RATS VIA A CYSTEINE ADDUCT IN HEMOGLOBIN

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (10) , 4254-4259
Abstract
The feasibility of monitoring doses of 4-aminobiphenyl (ABP) via adduction to Hb was investigated. Rats dosed with ABP (from 0.5-5 mg/kg) formed a stable covalent Hb:ABP adduct. Approximately 5% of a single dose was bound as Hb:ABP; chronic dosing led to an accumulation of the adduct to a level 30 times greater than that found after a single dose. Facile in vitro hydrolysis of the adduct regenerated ABP, allowing detection at the sub-ng level. Human Hb was also readily adducted, using N-hydroxy-ABP in vitro. The predominant site of adduction appeared to be the cysteine residue in Hb. The use of such adducts as dosimeters for arylamine exposures in humans is discussed.