TITRATION BY INDIRECT IMMUNOFLUORESCENCE OF ANTIBODY TO CRIMEAN HEMORRHAGIC FEVER IN A DROP OF CELL-SUSPENSION FROM INFECTED CELL-CULTURES

  • 1 January 1977
    • journal article
    • research article
    • No. 5,p. 606-608
Abstract
A comparatively simple method was developed and tested for the detection and titration of antibody to Crimean hemorrhagic fever virus (CHF) by indirect immunofluorescence on slides with previously prepared acetone-inactivated virus in drops of cell suspension from infected baby hamster kidney BHK-21 and 6619 cell cultures. The antibody titers determined by the indirect immunofluorescence procedure were 4- and 8-fold higher than those determined by the complement-fixation test. This opens the possibility of using noninfectious antigens of cell suspensions for detection or titration by the fluorescent antibody procedure of antibody to CHF virus which may be of importance to diagnostic laboratories which have no conditions for work with live virus.

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