Biochemical Changes and Incorporation of P32 in Separated Elements of the Hyperplastic Lesion of Fowlpox

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Abstract
It is known that fowlpox virus infection of chick skin results in conspicuous hyperplasia of the epithelium and thickening of the connective tissue due to hyperplasia and inflammatory changes. Normal control and fowlpox infected chick scalp were separated by trypsin into connective tissue and epithelium and with appropriate techniques the infected epithelial tissue was separated into surface and follicular epithelium (follicles). Comparison was made between normal epithelium and connective tissue and between normal and infected tissues with respect to certain lipids and incorporation of p32 into phospholipid. Distinct differences in phospholipid and total, free, and esterified cholesterol were found between normal epithelium and connective tissue. The above components were increased two- to threefold at some interval of time in both infected epithelium and connective tissue. The specific activities of phospholipid P from all tissues were approximate at 3 days but that of normal epithelium and connective tissue fell approximately 50% at 5 and 7 days. In contrast the incorporation of p32 in infected tissues was 1.5 to 1.7 times that of normal controls.

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