Electron Microscopy in Mammary Cancer Research12

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Abstract
Morphological details of B and C particles are quite variable when observed in thin sections, and in many cases individual particles cannot be identified as either B or C. Negative staining provides a more discriminatory technique, since the unique surface projections of B particles are revealed by this method, enabling them to be clearly distinguished from C particles or other debris. By use of this technique, types of virus-like particles were observed in human milk: 1) MS-1 with spikes identical to the spikes of mouse mammary tumor virus (MuMTV); 2) MS-2 with spikes which are not identical to those of MuMTV, and 3) MS-3 having heads and tails and a smooth outer membrane. Some human milks have a destructive effect on the morphology of MuMTV.

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