Microarray Analysis of Diurnal and Circadian-Regulated Genes in Arabidopsis
- 1 January 2001
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 13 (1) , 113-123
- https://doi.org/10.1105/tpc.13.1.113
Abstract
Plants respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24-hr period. To identify novel genes regulated in this way, we used microarrays containing 11,521 Arabidopsis expressed sequence tags, representing an estimated 7800 unique genes, to determine gene expression levels at 6-hr intervals throughout the day. Eleven percent of the genes, encompassing genes expressed at both high and low levels, showed a diurnal expression pattern. Approximately 2% cycled with a circadian rhythm. By clustering microarray data from 47 additional nonrelated experiments, we identified groups of genes regulated only by the circadian clock. These groups contained the already characterized clock-associated genes LHY, CCA1, and GI, suggesting that other key circadian clock genes might be found within these clusters.Keywords
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