Simultaneous HPLC Analysis of the Hypnotic Benzodiazepines Nitrazepam, Estazolam, Flunitrazepam, and Triazolam in Plasma
- 1 November 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 15 (6) , 319-322
- https://doi.org/10.1093/jat/15.6.319
Abstract
A new analytical methodology was developed for simultaneous high-performance liquid chromatographic quantitation of nitrazepam, estazolam, flunitrazepam, and triazolam in plasma. After addition of the internal standard nor-prazepam, biological samples and calibration standards were extracted at basic pH into diethyl ether-methylene chloride (2:1, v/v). The reconstituted extract was separated on a reversed-phase Nova Pak C18 column with acidic buffer (6mM)-acetonitrile-methanol (64:23:13, v/v) as mobile phase. The detection was performed at 242 nm. Within-run and day-to-day precision values were about 2–8%. The method is thus sensitive and reproducible for use in clinical and experimental toxicokinetic studies.Keywords
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