Derivatives of cyclosporin compatible with antibody-based assays: I. The generation of [125I]-labeled cyclosporin.
- 1 March 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 31 (3) , 459-462
- https://doi.org/10.1093/clinchem/31.3.459
Abstract
The immunosuppressive drug cyclosporin A, has been successfully iodinated to a specific activity of 300 Ci per gram. 125I-labeled cyclosporin and [3H]cyclosporin are nearly equivalent as tracers in a radioimmunoassay in producing standard lines (suppression by unlabeled cyclosporin) and in assigning values to clinical samples. In addition, the [125I]-labeled cyclosporin has greater than twice the sensitivity, and it is stable to long-term storage. Use of a [125I]-labeled cyclosporin tracer is more convenient, more reproducible, more precise, and easier than the tritiated-cyclosporin alternative in radioimmunoassay of this compound.Keywords
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