Insulin antagonises the growth hormone‐mediated increase in the activity of phosphatidate phosphohydrolase in isolated rat hepatocytes

Abstract
Rat hepatocytes were incubated in monolayer culture, under serum‐free conditions for 8 h. Rat growth hormone (up to 100 nM) increased the activity of phosphatidate phosphohydrolase by up to 41%. Insulin (500 pM or 35 nM), cycloheximide or actinomycin D reversed this effect. The ability of growth hormone to modify the effects of insulin is discussed in relation to the control of the phosphohydrolase activity and glycerolipid synthesis.

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