SYR2, A Gene Necessary for Syringomycin Growth Inhibition of Saccharomyces Cerevisiae
- 1 March 1996
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 142 (3) , 477-484
- https://doi.org/10.1099/13500872-142-3-477
Abstract
The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2 was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2 which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.Keywords
This publication has 40 references indexed in Scilit:
- Novel bioactive lipodepsipeptides from Pseudomonas syringae: The pseudomycinsFEBS Letters, 1994
- Yeast mutants affected in viability upon starvation have a modified phospholipid compositionYeast, 1993
- Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturaseGene, 1991
- [12] High-efficiency transformation of yeast by electroporationPublished by Elsevier ,1991
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Structure of syringotoxin, a bioactive metabolite of Pseudomonas syringae pv. syringaeFEBS Letters, 1990
- Structure of phytotoxin syringomycin produced by a sugar cane isolate of pseudomonas syringae pv. syringaeTetrahedron Letters, 1990
- The expression of two transcripts of the phosphodiesterase gene during the development of Dictyostelium discoideumDevelopmental Biology, 1987
- Bacterial phytotoxin, syringomycin, induces a protein kinase-mediated phosphorylation of red beet plasma membrane polypeptidesProceedings of the National Academy of Sciences, 1987
- Mechanism of Action of Pseudomonas syringae Phytotoxin, SyringomycinPlant Physiology, 1987