An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion.
- 1 February 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (2) , 645-649
- https://doi.org/10.1073/pnas.75.2.645
Abstract
Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.6.6) to synthesize an RNA at the origin [ori] of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.This publication has 17 references indexed in Scilit:
- Structure of the orgin of DNA replication of bacteriophage fd.Proceedings of the National Academy of Sciences, 1978
- Interaction of DNA with DNA‐Binding ProteinsEuropean Journal of Biochemistry, 1976
- A DNA fragment from the origin of single-strand to double-strand DNA replication of bacteriophage fd.Proceedings of the National Academy of Sciences, 1976
- Molecular Aspects of DNA Replication in Escherichia coli SystemsPublished by Springer Nature ,1976
- Procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatographyBiochemistry, 1975
- DNA Polymerase III Star Requires ATP to Start Synthesis on a Primed DNAProceedings of the National Academy of Sciences, 1973
- 5 s RNA Conformation. Studies of its Partial T1 Ribonuclease Digestion by Gel Electrophoresis and Two-dimensional Thin-layer ChromatographyJournal of Molecular Biology, 1972
- A DNA-Unwinding Protein Isolated from Escherichia coli: Its Interaction with DNA and with DNA PolymerasesProceedings of the National Academy of Sciences, 1972
- RNA Synthesis Initiates In Vitro Conversion of M13 DNA to Its Replicative FormProceedings of the National Academy of Sciences, 1972
- A Possible Role for RNA Polymerase in the Initiation of M13 DNA SynthesisProceedings of the National Academy of Sciences, 1971