Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats.
- 1 February 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (4) , 935-939
- https://doi.org/10.1073/pnas.84.4.935
Abstract
Laminin is a basement membrane-specific glycoprotein (800 kDa) consisting of three chains: A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. We have isolated two overlapping cDNA clones that span 5.9 kilobases and code for the entire B1 chain of mouse laminin. The nucleotide sequence of the clones reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. Analysis of the deduced protein sequence predicts that the B1 chain has seven distinct domains that include cysteine-rich repeats, alpha-helical, and globular structures. Part of the cysteine-rich region is homologous to epidermal growth factor and other proteins that contain epidermal growth factor-like repeats.This publication has 39 references indexed in Scilit:
- Localization of binding sites for laminin, heparan sulfate proteoglycan and fibronectin on basement membrane (type IV) collagenJournal of Molecular Biology, 1986
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- MYOSINAnnual Review of Biochemistry, 1984
- Neurite extension by peripheral and central nervous system neurons in response to substratum-bound fibronectin and lamininDevelopmental Biology, 1983
- Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursorNature, 1983
- Protease Resistance and Conformation of LamininEuropean Journal of Biochemistry, 1982
- Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrixJournal of Molecular Biology, 1981
- Hormonal induction of differentiation in teratocarcinoma stem cells: Generation of parietal endoderm by retinoic acid and dibutyryl cAMPCell, 1980
- Sequence organization of two recombinant plasmids containing genes for the major heat shock-induced protein of D. melanogasterCell, 1979
- Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteinsJournal of Molecular Biology, 1978