In Vivo 23 Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85
Open Access
- 1 September 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (9) , 4390-4392
- https://doi.org/10.1128/aem.67.9.4390-4392.2001
Abstract
Sodium gradients (ΔpNa) were measured in resting cells of Fibrobacter succinogenes by in vivo 23 Na nuclear magnetic resonance using Tm(DOTP) 5− [thulium(III) 1,4,7,10-tetraazacyclododecane- N ′, N ′′, N ′′′-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a ΔpNa of −55 to −40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na + ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).Keywords
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