CHANGES IN MEMBRANE GANGLIOSIDES - DIFFERENTIATION OF HUMAN AND MURINE MONOCYTIC CELLS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 58  (2) , 125-131
Abstract
Membrane ganglioside changes in murine peritoneal macrophages and the human promyelocytic leukemia cell line HL-60 were assessed by 2-dimensional TLC. C3H/HeJ mice responded to protein-containing endotoxin but were hyporesponsive to protein-free endotoxin preparations. Compared to unstimulated resident cells, protein-containing endotoxin produced an alteration in the C3H/HeJ macrophage ganglioside pattern, whereas protein-free endotoxin did not. In comparison, differentiation of HL-60 cells to a neutrophil-like cell by dimethylsulfoxide gave a ganglioside pattern similar to unstimulated HL-60 cells. Differentiation of HL-60 cells by phorbol myristate acetate to macrophage-like cells results in a large increase in the monosialoganglioside GM3. Discrete ganglioside changes occur in murine monocytes and HL-60 cells upon induction to cells with increased macrophage functions.