HUMAN GRANULOSA CELLS SYNTHESIZE LOW MOLECULAR WEIGHT INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN

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Abstract
Human follicular fluid contains insulin-like growth factor 1 (IGF-1) and its low molecular weight binding protein (IGF-BP). We studied the synthesis of IGF-BP by the granulosa cells obtained after ovarian hyperstimulation for in vitro fertilization. The granulosa cells were cultured for 72 hours in Ham''s F-10 medium supplemented with 10% fetal calf serum (FCS). Samples of the culture medium were collected every 24 hours. The IGF-BP concentration in culture medium increased from 1.2 to 2.1 .mu.g/l at 48 h and to 3.3 .mu.g/l at 72 h. De novo synthesis of IGF-BP was shown by incorporation of labeled methionine into immunoreactive IGF-BP, as detected by SDS polyacrylamide electrophoresis (PAGE) and fluorography. Our results demonstrate synthesis of IGF-BP in the human ovary.

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