Separation of Pancreatic Enzymes by Gel Filtration.

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Abstract
The use of gel filtration on Sephadex columns for fractionation of enzymes in pancreatic extracts has been studied. On gels with porous structures an adequate separation of lipase, proteolytic enzymes and amylase is obtained, indicating a simple and convenient method for analytical and preparative purposes. The behaviour of individual enzymes under various experimental conditions is discussed. Amylase is slightly retained on the column due to an interaction with the gel matrix, and pancreatic lipase is separated into two fractions, probably consisting of polymer and monomer forms. The molecular weight of the monomer is estimated to be approximately 35,000.

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