Cellular fatty acids derived from normal alkanes byCandida rugosa
- 1 January 1980
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 20 (7) , 449-457
- https://doi.org/10.1002/jobm.3630200704
Abstract
In an attempt to examine the specific metabolic relationship between the alkane substrates and lipid products the pattern of formation of cellular lipid, especially fatty acids, has been studied in the course of incubation of cells of C. rugosa with n-alkanes in mineral medium. C18:2, C18:1, C16:1 and C16:0 fatty acids were the major products formed, irrespective of the odd or even chain length of the alkane used as substrate. These unsaturated fatty acids reached a maximum in the stationary phase. In the case of cells grown on odd-chain n-alkanes (from C11 to C19), the proportion of odd-chain cellular fatty acids were markedly high, reaching 77--88% in the n-pentadecane and heptadecane-grown cells. The resulting acids are then metabolized by beta-oxidation or inserted directly or after elongation with C2-units into the cellular fatty acids. The total lipid content of n-alkane (n-C10-C20) grown cells in the stationary phase was 16.3--19.0% of th dry cell weight, which was about three times as much as that of glucose-grown cells. The chain length of alkane substrates had a significant effect on the lipid content. Ergosterol production from n-hexadecane was twice higher than that from glucose.Keywords
This publication has 13 references indexed in Scilit:
- CELLULAR FATTY ACID COMPOSITION IN GRAM-NEGATIVE OBLIGATELY ANAEROBIC RODSThe Journal of General and Applied Microbiology, 1979
- MICROBODIES OF THE HYDROCARBON-ASSIMILATING YEAST CANDIDA RUGOSAThe Journal of General and Applied Microbiology, 1977
- Coagulase and Heat-resistant Nuclease ProducingStaphylococcus epidermidisStrains from AnimalsJournal of Applied Bacteriology, 1975
- The intracellular polysaccharide ofCandida rugosa grown on hydrocarbonJournal of Basic Microbiology, 1974
- Monosaccharide formation from hydrocarbon byCandida rugosaJournal of Basic Microbiology, 1974
- Effects of Chain-length of Alkane Substrate on Fatty Acid Composition and Biosynthetic Pathway in Some Candida YeastsAgricultural and Biological Chemistry, 1973
- Enzymatic conversion of l-decene to decyl alcohol byCandida rugosa JF 101Journal of Basic Microbiology, 1971
- Comparative lipid content ofCandida lipolytica grown on glucose and onn-hexadecaneAntonie van Leeuwenhoek, 1968
- Effect of Substrate on the Fatty Acid Composition of Hydrocarbon-utilizing MicroorganismsJournal of Bacteriology, 1967
- Microbial Incorporation of Fatty Acids Derived From n-Alkanes Into Glycerides and WaxesApplied Microbiology, 1964