The Comparison of Elution Profiles of Polychlorinated Biphenyls and Fats in Various Gel Permeation Chromatographic Systems

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Abstract
An optimisation of gel permeation chromatography (GPC) is presented for separation of polychlorinated biphenyls (PCBs) from fats. The results of testing of Bio Beads S-X3 gel eluted with different mobile phases (mixtures of chloroform and cyclohexane) are evaluated with respect to the use of GPC as a single, rapid and efficient clean-up technique suitable for routine analyses of PCBs. Relations between theoretical assumptions and achieved results are discussed. Although the elution profiles for individual PCB congeners differed, various tested fats eluted in similar volumes. Several of investigated systems can be used for isolation of PCBs from fats. Nevertheless, for practical reasons a system with chloroform as a single mobile phase, in which PCBs were eluted in the lowest volume as a narrow fraction, has been chosen.