Microinjection of chemical carcinogens into small fish embryos: exocrine pancreatic neoplasm in Fundulus grandis exposed to N-methyl-A/'-nitro-N-nitrosoguanidine
- 1 January 1988
- journal article
- research article
- Published by Inter-Research Science Center in Diseases of Aquatic Organisms
- Vol. 5 (2) , 101-105
- https://doi.org/10.3354/dao005101
Abstract
Embryos of gulf killifish Fundulus grandis were injected with N-methyl-N-nitro-N-nitrosoguanidine (MNNG). Retention of carcinogen in the egg immediately after injection was highly variable. Hatching percentages of MNNG-injected and uninjected embryos were similar, but survival of MNNG-injected fish during the first week after hatching was less than half of the survival of controls. One of 3 MNNG-exposed fish examined after 20 wk had a pancreatic acinar cell carcinoma, a type of neoplasm seldom found in fish. No neoplasms were recorded in controls nor in MNNG-exposed fish examined after 34 wk. The neoplasm was invasive and had a high mitotic index, but no metastases were found. The acinar cell origin of the tumor was confirmed by ultrastructural examination of tissue reprocessed from paraffin into epoxy resin.Keywords
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