Genetic Manipulation of Mycobacterium tuberculosis
Open Access
- 1 August 2007
- journal article
- unit
- Published by Wiley in Current Protocols in Microbiology
- Vol. 6 (1) , 10A.2.1-10A.2.21
- https://doi.org/10.1002/9780471729259.mc10a02s6
Abstract
This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis, including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed. Curr. Protoc. Microbiol. 6:10A.2.1-10A.2.21. © 2007 by John Wiley & Sons, Inc.Keywords
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